DOI: http://dx.doi.org/10.18203/issn.2454-5929.ijohns20163472

Level of serum lactate dehydrogenase in oral submucous fibrosis

K. Mallikarjuna Swamy, Arati Ganiger

Abstract


Background: Oral cancer is one of the most common form of malignancies in India. In many cases it develops at the site of premalignant lesion. Of all oral premalignant conditions, oral submucous fibrosis (OSMF) is of greater concern because of its disabling nature and relative greater chances of malignant transformation. Transformation of normal tissue to premalignant lesion and further to oral cancer results in alteration in glycolytic pathway and hence the lactate dehydrogenase (LDH) levels. The aim of this study was to estimate the LDH levels in serum of subjects with OSMF and to compare them with healthy controls and to correlate the relationship between pathogenesis of OSMF and the LDH enzyme.

Methods: It is a case control study. The study included 40 diagnosed cases of OSMF and 40 matched healthy controls. Venous blood of 3 ml was collected in both cases and controls. Serum was separated by centrifugation and LDH was estimated by using standard kits. Statistical analysis was done using student ‘t’ test. Pearson's correlation was performed to establish the relationship between study variables.  

Results: It was observed that serum LDH levels were significantly increased in cases of OSMF as compared to controls (p <0.005).

Conclusions: Serum LDH was significantly increased in OSMF and can be used as a valuable biochemical marker in prognosis of OSMF.


Keywords


OSMF, LDH, Premalignant, Oral cancer, Glycolytic pathway

Full Text:

PDF

References


Prabhu SR, Johnson NW, Daftary DK, Wilson DF. Oral Diseases in the Tropics. 1st edition. USA: Oxford University Press; 1992.

Wood NK, Goaz PW. Differential Diagnosis of Oral and Maxillofacial Lesions. 5th ed. St. Louis, Missouri: Elsevier; 2006: 587.

Khan Z. An overview of oral cancer in indian subcontinent and recommendations to decrease its incidence. Available from : http://www.webmed

central.com/article view/3626. Accessed on August 6, 2015.

World Health Organisation (WHO). Guide to epidemiology and diagnosis of oral mucosal diseases and conditions. Community dent Oral epidemiol. 1980;8:1-26.

Lemmer J, Singh B. Formation of vesicles in oral cavity submucous fibrosis. Acta path Microbial Scand. 1967;70:161-73.

Pinborg JJ, Singh B. Formation of vesicles in oral submucous fibrosis. Acta path Microbial scand. 1967;70:161-73.

Sirsat SM, Pindborg JJ. Subepithelial changes in oral submucous fibrosis. Acta path Scand. 1967;70:161-73.

Wahi PN, Luthra UK, Kapur VL. Submucous fibrosis of the oral cavity: histomorphological studies. Br J cancer. 1966;20:676-87.

Lingen MW, Kalmar JR, Karrison T, Speight PM. Critical evaluation of diagnostic aids for the detection of oral cancer. Oral Oncol. 2008;44:10-22.

Joshi PS, Chougule M, Dudanakar M, Golgire S. Comparison between salivary and serum lactate dehydrogenase levels in patients with oral leukoplakia and oral squamous cell carcinoma-A pilot study. Int J Oral Maxillofac Pathol. 2012;3:7-12.

Anuradha CD, Devi CS. Studies on enzymes of clinical signifcance in oral sub mucous fibrosis. J Clin Biochem Nut. 1998;24:45-52.

Kamath VV, Satelur K, Komali Y. Biochemical markers in oral sub mucous fibrosis: A review and update. Dent Res J (Isfahan). 2013;10:576-84.

T-Tomity I, Takács O. Investigations on the distribution of serum LDH isoenzymes of patients with carcinoma laryngis. Laryng Rhino Otol (Stug). 1979;58:916-9.

Giannoulaki EE, Kalpaxis DL, Tentas C, Fessas P. Lactate dehydrogenase isoenzyme pattern in sera of patients with malignant diseases. Clin Chem. 1989;35:396-9.

Shklar G. Enzyme histochemistry of human oral carcinoma. Oral Surg Oral Med Oral Pathol. 1966;21:764-9.

Gorogh T, Eickbohm JE, Ewers R, Lippert B. Lactate dehydrogenase isoenzymes in squamous cell carcinomas of the oral cavity. J Ora Pathol Med. 1990;19:56-9.

Dreyfuss AI, Clark JR, Andersen JW. Lipid-associated sialic acid squamous cell carcinoma antigen, carcinoembryonic antigen and lactic dehydrogenase levels as tumor markers in squamous cell carcinoma of the head and neck. Cancer. 1992;70:2499-503.

Liaw CC, Wang CH, Huang JS, Kiu MC, Chen JS, Chang HK. Serum lactate dehydrogenase level in patients with nasopharyngeal carcinoma. Acta Oncol. 1997;36:159-64.

Searcy RL. Diagnostic Biochemistry. New York, NY: McGraw-Hill; 1969.

Tietz. Textbook of Clinical Chemistry and Molecular Diagnostics. In: Burtis CA, Ashwood, ER, Bruns DE. 5th edition. WB Saunders Comp; 2012.

Henry RJ, Chiamori N, Golub OJ, Berkman S. Revised spectrophotometric methods for the determination of glutamic oxalacetic transaminase, glutamic pyruvic transaminase, and lactic acid dehydrogenase. Am J Clin Path. 1960;34(341):381-98.

Lum G, Gambino SR. A comparison of serum versus heparinized plasma for routine chemistry tests. Am J Clin Pathol. 1974;61(108):108-13.

Bergmeyer HW. Methods of Enzymatic Analymatic Analysis, 2nd edition. Verlog Chemie; 1965.

Young DS. Effects of Drugs on Clinical Laboratory Tests. Third Edition. 1990;3:221-4.

Hong SH, Roh SY, Ko YH, Won HS, Lee MA, Woo IS, et al. Prognostic significance of glycolytic metabolic change related to HIF-1alpha in oral squamous cell carcinomas. Korean J Patho. 2010;44:360-9.

Drent M, Cobben NA, Henderson RF, Wouters EF, Van Dieijen-Visser M. Usefulness of lactate dehydrogenase and its isoenzymes as indicators of lung damage or inflammation. Eur Respir J. 1996;9:1736-42.