Tuberculous mycobacteria bacilli fluorescence and compare with Ziehl- Neelsen stain in fine-needle aspiration cytology of tubercular lymphnode
DOI:
https://doi.org/10.18203/issn.2454-5929.ijohns20160956Keywords:
Tuberculosis, Mycobacterium tuberculosis, AFB, ZN staining, AR staining, AFAbstract
Background: Tuberculosis is infectious disease caused by Mycobacterium tuberculosis. There are various methods for diagnosis of tuberculosis such as direct clinical material examination of tubercular bacilli by Ziehl – Neelsen (ZN) staining, demonstration of tubercular bacilli by auramine – rhodamine (AR) staining and autofluorescence (AF).
Methods: Present study was done clinically suspected tubercular patients. All received samples Zn stain, fluorescent stain and PAP stain were applied.
Results: Among the clinically suspected patients 88 was diagnosed with tuberculosis. Female preponderance was noted accounting for 60.23% (53/88) of cases. Of the 88 aspirates, the smear positivity for acid fast bacilli (AFB) on the ZN method was 37.5% (33/88) while the positivity increased to 81.82% (72/88) on the AR fluorescent method and 86.36% (76/88) on AF.
Conclusions: AF staining is more sensitive than the auramine – rhodamine fluorescent and ZN staining in demonstration of mycobacterium bacilli in fine needle aspiration cytology of tubercular lymphnode.Metrics
References
Dandapat MC, Mishra BM, Dash SP, Kar PK. Peripheral lymph node tuberculosis: A review of 80 cases. Br J Surg. 1990;77(8):911-2.
Lau SK, Kwan S, Lee J, Wei WI. Source of tubercle bacilli in cervical lymph nodes: A prospective study. J Laryngol Otol. 1991;105(7):558-61.
David HL: bacteriology of the mycobacterioses. US department of health, education and welfare, PHS, CDC, Washington DC; 1976.
Daniel TM. Rapid diagnosis of tuberculosis: Laboratory techniques applicable in developing countries. Rev Infect Dis. 1989;2(Suppl 2):471-8.
Balows A, Hausler WJ, Hermann KL, Shadomy HJ. Manual of clinical Microbiology. 5th ed. Washington: D.C: American society for Microbiology 1991:308-11.
Savic B, Sjobring U, Alugupalli S, Larsson L, Miorner H. Evaluation of polymerase chain reaction, tuberculostearic acid analysis, and direct microscopy for the detection of Mycobacterium tuberculosis in sputum. J Infect Dis. 1992;166(5):1177-80.
Jain A, Bhargava A, Agarwal SK. A comparative study of two commonly used staining techniques for acid fast bacilli in clinical specimens. Indian Journal of Tuberculosis. 2002;49:161-2.
Wright CA, van Zyl Y, Burgess SM, Blumberg L, Leiman G. Mycobacterial autofluorescence in papanicolau stained lymph node aspirates: a glimmer in the dark? Diagnostic Cytopathology. 2004;30(4):257-60.
Wright CA, M. Burg MV, Geiger D, Noordzij JG, Burgess SM,Marais BJ. Diagnosing mycobacterial lymphadenitis in children using fine needle aspiration biopsy: cytomorphology, ZN staining and autofluorescence –making more of less. Diagnostic Cytopathology. 2008;36(4):245-51.
Joshi P, Singh M, Bhargava A, Singh M, Mehrotra R. Autofluorescence- an important ancillary technique for the detection of Mycobacterium tuberculosis: revisited. Diagnostic Cytopathology. 2012;41(4):330-4.
Thakur B, Mehrotra R, Nigam JS. Correlation of various techniques in diagnosis of tuberculous lymphadenitis on fine needle aspiration cytology. Pathology Research International. 2013;2013: http://dx.doi.org/10.1155/2013/824620.